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mouse anti p erk 1 2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti p erk 1 2
    Mouse Anti P Erk 1 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2439 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+p+erk+1+2/p-ERK+1%2F2+Antibody/pm41914021-63-79-84
    Average 96 stars, based on 2439 article reviews
    mouse anti p erk 1 2 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Glucose shields RINm5F beta cells against arsenic-induced apoptosis
    Article Snippet: Proteins were transferred to PVDF membranes using a semi-dry transfer system (Bio-Rad, USA) at 20 V for 60 min. Membranes were blocked with TBS-tween containing 4% Blotto non-fat dry milk (Santa Cruz Biotechnology, sc-2325, USA) for 1 h at room temperature. .. Primary antibodies were diluted in TBS-Tween 20 with 4% milk and incubated overnight at 4 °C with rabbit anti-p-Akt (Ser473) (#9271, DF 1:500, Cell Signaling), rabbit anti-Akt (#9272, DF 1:2000, Cell Signaling), rabbit anti-p-P70S6K (Thr389) (#9205, DF 1:500, Cell Signaling), rabbit anti-P70S6K (#9202, DF 1:1000, Cell Signaling), rabbit anti-p-IRS1 (S1101) (#2385, DF 1:1000, Cell Signaling), rabbit anti-IRS1 (#2382, DF 1:1000, Cell Signaling), mouse anti-p-ERK 1/2 (T202/Y204.22 A) (SC-136521, DF 1:200, Santa Cruz Biotechnology), mouse anti-ERK 1/2 (SC-514302, DF 1:1000, Santa Cruz Biotechnology), rabbit anti-cleaved caspase-3 (N175) (#9661, DF 1:1000, Cell Signaling), mouse anti-BcL-xL (SC-8392, DF 1:1000, Santa Cruz Biotechnology), rabbit anti-Bax (SC-493, DF 1:1000, Santa Cruz Biotechnology), rabbit anti-calpain-1 (#2556, DF 1:1000, Cell Signaling), rabbit anti-calpain-2 (ab126600, DF 1:1000, Abcam), rabbit anti-calpain-10 (ab28226, DF 1:1000, Abcam), rabbit anti-αII spectrin (BML-FG 6090 − 0100, DF 1:1000, ENZO).Secondary antibody: Peroxidase-conjugated mouse anti-rabbit IgG (light chain specific, Jackson ImmunoResearch #211-032-171, DF 1:6000) and peroxidase-conjugated affinipure goat anti-mouse IgG (Jackson ImmunoResearch #115-035-146; DF 1:4000). .. Protein bands were detected using the ECL Prime kit (Amersham, UK) and visualized with a Li-Cor C-Digit scanner.

    Article Title: Panacis Quinquefolii Radix Polysaccharides Alleviate Depressive-Like Behaviors in Chronic Unpredictable Mild Stress-Induced Mice by Suppressing Complement C1Q/C3-Mediated Microglial Synaptic Pruning and Modulating Gut Microbiota.
    Article Snippet: .. After blocking with 5% skim milk for 1 h and washing with TBST, the membranes were incubated overnight with various primary antibodies: Rabbit antiCD11b (Abcam, ab133357, 1:1000), mouse anti- C3 (Proteintech, 66157–1- ig, 1:1500), rabbit anti- C1Q (ABclonal, A24519, 1:2000), mouse anti- Tubulin (Fude Biotechnology, FD0064, 1:2500), rabbit anti- p- STAT3 (Abcam, ab32143, 1:5000), rabbit anti- STAT3 (Cell Signaling Technology, 4904 T, 1:2000), rabbit anti- p- P65 (ImmunoWay, YP0191, 1:2000), rabbit anti- P65 (Cell Signaling Technology, 8242 T, 1:2000), mouse anti- p- ERK 1/2 (Santa Cruz Biotechnology, SC- 81492, 1:500), and mouse anti- ERK 1/2 (Santa Cruz Biotechnology, SC- 514302, 1:500). .. Following additional washes with TBST, they were incubated with HRP- conjugated secondary antibodies, Goat anti- mouse IgG (H + L) HRP (Fude Biotechnology, FDM007, 1:4000) and Goat anti- rabbit IgG (H + L) HRP (Fude Biotechnology, FDR007, 1:4000) for 2 h. Chemiluminescence detection was performed using an ECL kit, and protein levels were normalized to tubulin, with quantification done using Image J software.

    Article Title: Glucose shields RINm5F beta cells against arsenic-induced apoptosis.
    Article Snippet: Proteins were transferred to PVDF membranes using a semi-dry transfer system (Bio-Rad, USA) at 20 V for 60 min. Membranes were blocked with TBS-tween containing 4% Blotto non-fat dry milk (Santa Cruz Biotechnology, sc-2325, USA) for 1 h at room temperature. .. Primary antibodies were diluted in TBSTween 20 with 4% milk and incubated overnight at 4 °C with rabbit anti-p-Akt (Ser473) (#9271, DF 1:500, Cell Signaling), rabbit anti-Akt (#9272, DF 1:2000, Cell Signaling), rabbit anti-p-P70S6K (Thr389) (#9205, DF 1:500, Cell Signaling), rabbit anti-P70S6K (#9202, DF 1:1000, Cell Signaling), rabbit anti-p-IRS1 (S1101) (#2385, DF 1:1000, Cell Signaling), rabbit anti-IRS1 (#2382, DF 1:1000, Cell Signaling), mouse anti-p-ERK 1/2 (T202/ Y204.22 A) (SC-136521, DF 1:200, Santa Cruz Biotechnology), mouse anti-ERK 1/2 (SC-514302, DF 1:1000, Santa Cruz Biotechnology), rabbit anti-cleaved caspase-3 (N175) (#9661, DF 1:1000, Cell Signaling), mouse anti-BcL-xL (SC-8392, DF 1:1000, Santa Cruz Biotechnology), rabbit anti-Bax (SC-493, DF 1:1000, Santa Cruz Biotechnology), rabbit anti-calpain-1 (#2556, DF 1:1000, Cell Signaling), rabbit anti-calpain-2 (ab126600, DF 1:1000, Abcam), rabbit anti-calpain-10 (ab28226, DF 1:1000, Abcam), rabbit anti-αII spectrin (BML-FG 6090 − 0100, DF 1:1000, ENZO).Secondary antibody: Peroxidase-conjugated mouse anti-rabbit IgG (light chain specific, Jackson ImmunoResearch #211-032-171, DF 1:6000) and peroxidase-conjugated affinipure goat antimouse IgG (Jackson ImmunoResearch #115-035-146; DF 1:4000). .. Protein bands were detected using the ECL Prime kit (Amersham, UK) and visualized with a Li-Cor C-Digit scanner.

    Blocking Assay:

    Article Title: Panacis Quinquefolii Radix Polysaccharides Alleviate Depressive-Like Behaviors in Chronic Unpredictable Mild Stress-Induced Mice by Suppressing Complement C1Q/C3-Mediated Microglial Synaptic Pruning and Modulating Gut Microbiota.
    Article Snippet: .. After blocking with 5% skim milk for 1 h and washing with TBST, the membranes were incubated overnight with various primary antibodies: Rabbit antiCD11b (Abcam, ab133357, 1:1000), mouse anti- C3 (Proteintech, 66157–1- ig, 1:1500), rabbit anti- C1Q (ABclonal, A24519, 1:2000), mouse anti- Tubulin (Fude Biotechnology, FD0064, 1:2500), rabbit anti- p- STAT3 (Abcam, ab32143, 1:5000), rabbit anti- STAT3 (Cell Signaling Technology, 4904 T, 1:2000), rabbit anti- p- P65 (ImmunoWay, YP0191, 1:2000), rabbit anti- P65 (Cell Signaling Technology, 8242 T, 1:2000), mouse anti- p- ERK 1/2 (Santa Cruz Biotechnology, SC- 81492, 1:500), and mouse anti- ERK 1/2 (Santa Cruz Biotechnology, SC- 514302, 1:500). .. Following additional washes with TBST, they were incubated with HRP- conjugated secondary antibodies, Goat anti- mouse IgG (H + L) HRP (Fude Biotechnology, FDM007, 1:4000) and Goat anti- rabbit IgG (H + L) HRP (Fude Biotechnology, FDR007, 1:4000) for 2 h. Chemiluminescence detection was performed using an ECL kit, and protein levels were normalized to tubulin, with quantification done using Image J software.



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